Category Archives: HPLC

How To Review HPLC Chromatograms in Empower

Here is a brief video on how to quickly navigate through empower to review and process your acquired HPLC data.  This can also be done in real time.  (Be sure to watch in 720p HD).

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How To Set Up an HPLC Run

Empower is by far the most commonly used HPLC/UPLC software.  Here is a brief video on how to set up and start an automated run.

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HPLC 101

This Royal Society of Chemistry vid is the best I’ve seen to describe chromatographic principles within the context of HPLC.  According to the standard handbook, the money currently spent annually on HPLC exceeds that of any other analytical technique.

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3 Quicktips For Reviving A Dormant LC

Software was recently attained and installed on an Agilent 1200 Series LC after being down for quite some time, although this didn’t solve the problem entirely.  If you encounter the same, here is everything you need to know about how … Continue reading

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How To Get Rid Of Air In An HPLC

Air is kryptonite to an HPLC pump.  Although, it is a common problem when you’re delivering liquids at such high pressures.  Air can easliy get in the lines and into your column if you’re not careful.  At that point you … Continue reading

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Purify While You Sleep: HPLC Autosampling

SJU now has a Waters 717 Autosampler connected to a 600 HPLC pump and controller, thereby eliminating the need to manually inject samples.  With simple programming, up to 96 prepared vials can be injected and analyzed without a chemist present. … Continue reading

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